单采血小板在储存过程中的功能变化.docVIP

精品论文 参考文献 单采血小板在储存过程中的功能变化 陈映 左宇梅 李俊 解明娟 冷彩霞 　　(四川省什邡市人民医院 四川 什邡 618400) 　　【摘要】 目的:评估体外储存对单采血小板生理功能的影响。方法:选择健康献血者50名，经由血站采集制备50袋单采血小板，分别在储存的第1天、第2天、第3天、第4天、第5天(D1、D2、D3、D4、D5)检测它们的代谢功能(乳酸、乳酸脱氢酶、葡萄糖、pH)、聚集功能(聚集率)、活化功能(CD62P)、低渗休克反应、血小板计数等相关指标，统计分析各项指标的变化。结果:随着储存时间的延长，血小板计数及低渗休克反应没有明显变化(Pgt;0.05)，pH值、GLU及血小板聚集率明显下降(Plt;0.05)，LDH、LA及CD62P表达明显增强(Plt;0.05)。结论:随着储存时间的延长，单采血小板的计数和低渗休克反应没有变化，但是其活化增强，代谢功能、聚集功能发生明显变化。 　　【关键词】 单采血小板；活化功能；聚集功能；代谢功能 　　【中图分类号】R55 【文献标识码】A 【文章编号】2095-1752（2016）10-0060-02 　　Functional changes of platelets during storage Chen ying, Zou Yumei, Li Jun,Xie Mingjuan, Leng Caixia. 　　The Peoplersquo;s Hospital of Shifang City, Sichuan Province, Shifang 618400, China 　　【Abstract】Objective To evaluate the effects of in vitro storage on the physiological function of platelets in vitro. Methods Healthy blood donors, 50, via blood collection system by 50 bags of apheresis platelets, respectively in storage on day 1, day 2, day 3, the fourth day, the 5 day (D1, D2, D3, D4, D5) detection of their metabolic functions (lactic acid, lactate dehydrogenase, glucose, pH) and aggregation (aggregation), activation function (CD62P) and low permeability shock response and platelet counts related indicators. Results With the extension of storage time, the platelet count and low osmotic shock response did not change significantly (Pgt;0.05), pH, GLU and platelet aggregation rate decreased significantly (LDH), LA, Plt;0.05 and CD62P expression were significantly enhanced (Plt;0.05). Conclusion With the extension of storage time, the count and the low osmotic shock response of the single - collected platelet had no change, but the activation enhanced, the metabolic function and the aggregation function changed significantly. 　　【Key words】Apheresis platelets; Activation function; Aggregation function; Metabolic function 　　血小板是血液有形成分中相对密度最小的一种细胞组分，其主要生理功能为止凝血。目前，血小板制剂的制备方法有手工法和单采血小板[1]。当前我国主要从血小板、白细胞和红细胞的数量方面控制血小板制剂的质量，没有考虑血小板的功能