aqp-4、crp及vegf在大鼠脑出血后的表达表达与炎症反应的相关性研究.docVIP

精品论文 参考文献 AQP-4、CRP及VEGF在大鼠脑出血后的表达表达与炎症反应的相关性研究 贵阳中医学院第一附属医院神经外科 贵州 贵阳 550001 基金项目： 贵州省卫生计生委科技基金，gzwjkj2015-1-059 贵州省科技厅联合基金，黔科合LH字[2015]7281 贵阳市科技计划项目[项目编号：筑科合24)号] 贵阳市高层次创新型青年卫生人才基金项目(2013筑卫科技合同字创9号) 【摘要】目的 探讨水通道蛋白-4（AQP-4）、C-反应蛋白（CRP）及血管内皮生长因子（VEGF）在大鼠脑出血后的表达与炎症反应的相关性。方法将90只健康大鼠分为实验组72只。对照组18只。实验组72只，采用自体血制作尾状核脑出血模型，观察大鼠术后行为变化，分别在术后6h、24h、48h、3d、7d及14d处死动物。对照组18只，采用相同方法注入等量生理盐水。HE染色观察组织病理变化，ELISA法检测血清CRP、VEGF表达，免疫组织化学方法检??AQP-4蛋白的表达。结果 实验组Longa评分6h及14d高于对照组(P＜0.05)，24h、48h、3d及7d评分明显高于对照组(P＜0.01)。AQP-4、CRP在6h即升高，24h、48h、3d、7d明显升高，VEGF的表达随出血时间的延长而增加，3d明显增高，7d达高峰，对照组：术后CRP、VEGF无明显增高，AQP-4术后3d内升高，7d、 14d升高不明显，差异具有显著意义(P＜0.01)。结论 脑出血后AQP-4、CRP、VEGF呈现过度表达，其动态变化与炎症反应的相关性具有正相关性。推没三者以不同的方式参与脑出血后损伤修复的炎症反应。 Study on Correlation of AQP-4, CRP and VEGF Expression with Inflammatory Response in Rats after Intracranial Hemorrhage Zhang Bo；Wu Huajun；Zhong Zhijun；et al Department of Neurosurgery, the First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine (Guiyang, Guizhou) 550001 【Abstract】 Objective To investigate the correlation of aquaporin-4 (AQP-4), C-reactive protein (CRP) and vascular endothelial growth factor (VEGF) expression with infammatory response in rats after intracranial hemorrhage (ICH). Methods A total of 90 healthy rats were divided into treatment group (72 rats) and control group (18 rats). Seventy-two rats in the treatment group used the autologous blood to make the caudatum ICH model in order to investigate postoperative behavioral changes of rats. Those animals were executed at 6h, 24h, 48h, 3d and 7d, respectively after operation. Eighteen rats in the control group were infused the equivalent normal saline (NS) using the same method. Pathological changes of tissues were observed by HE staining; serum CRP and VEGF expression was tested by ELISA; and AQP-4 protein exression was tested by immunohistochemistry. Results Longa scores at 6h and 14d in the treatment group were higher than those of control group (P＜0