P27蛋白在宫颈鳞状细胞癌中表达.docVIP

P27蛋白在宫颈鳞状细胞癌中表达 　 作者：毛荣军，李启明，房慧琳，王桂芳 【摘要】 目的 探讨P27蛋白表达与宫颈鳞状细胞癌发生、发展的关系。方法 采用SP法免疫组化分别检测宫颈鳞状细胞癌、癌旁高级别鳞状上皮病变、癌旁正常鳞状上皮及非癌旁正常鳞状上皮组织中P27蛋白的表达。结果 宫颈鳞状细胞癌、癌旁高级别鳞状上皮病变、癌旁正常鳞状上皮及非癌旁正常鳞状上皮组织中P27阳性表达率分别为35.00%(21/60)、38.89%（14/36）、77.78%(42/54)、80.00%(8/10)，其中宫颈鳞状细胞癌及癌旁高级别鳞状上皮病变P27阳性表达率与癌旁及非癌旁正常宫颈鳞状上皮相比差异均有显著性(Plt;0.05或0.01)。P27阳性表达率下降与组织学分级、肿瘤大小、肿瘤浸润及淋巴转移有关(P均lt;0.01)，与年龄无关（Pgt;0.05）。结论 P27在宫颈鳞状细胞癌的发生、发展过程中起重要的作用，体现在P27蛋白表达降低或缺失时易导致宫颈鳞状细胞癌的发生和转移，因此检测P27蛋白表达可作为宫颈鳞状细胞癌的发生、发展及预后评估的一个有用指标。 【关键词】 宫颈肿瘤；癌，鳞状细胞；P27蛋白 　　Abstract: Objective To investigate the relationship between the expression of P27 protein and the oncogenesis and development of cervical squamous carcinoma (CSC). Methods The expression of P27 was detected by using SP immunohistochemical staining in paraffin sections from cases of CSC, high-grade squamous intraepithelial lesion (HSIL) and corresponding normal squamous tissues (CNST) closely adjacent to carcinoma, and other normal squamous tissues (ONST). Results The positive rates of P27 protein expression in SCS, HSIL, CNST and ONST were 35% (21/60), 38.89% (14/36), 77.78% (42/54) and 80% (8/10), respectively. The comparison of positive rates among SCS, HSIL and CNST, ONST showed significant differences (Plt;0.05 or 0.01). The decreased expression of P27 protein was associated with histologic grading, tumor size, invasion and metastasis of SCS (all Plt;0.01), and it was not associated with age (Pgt;0.05). Conclusion P27 protein might play an important suppressing role in the genesis and development of CSC, the down-regulated expression of CSC might easily lead to lymphnode metastasis and tumorous invasion, expression of P27 could be helpful in predictin the biological behavior and prognosis of CSC. 　　Key words: cervical neoplasms; carcinoma, squamous cell; P27 protein 宫颈鳞状细胞癌是我国女性最常见的恶性肿瘤之一，多种因素长期的共同作用促进了宫颈鳞状细胞癌的发生与发展，在此过程中涉及多基因、多分子水平的改变，其中包括癌基因的激活和抑癌基因的失活，导致了细胞无限制地生长，从而引起肿瘤的发生。在细胞生长过程中，对G1期的调控是细胞周期的关键调控点。P27是细胞周期G1 期重要的负向调控因子，是近年来发现被认为是一种新的候选抑癌基因。目前，它在宫颈鳞状细胞癌中的研