P27及skp2在肝细胞癌中表达及其意义.docVIP

P27及skp2在肝细胞癌中表达及其意义 　【摘要】 　　目的 探讨肝细胞癌P27和skp2蛋白的表达情况与临床病理意义。方法 用免疫组化二步法检测P27和skp2在60例肝细胞癌及20例癌旁组织中的表达。结果 60例肝细胞癌P27的阳性表达率为23%(14/60)，癌旁组织为80%(16/20)，60例肝细胞癌高分化癌阳性表达率为47%(7/15)，低分化癌为16%(7/45)。P27在肝细胞癌中的表达明显低于癌旁组织，P27蛋白的表达随病理分级增高而降低。60例肝细胞癌skp2阳性表达率为47%(28/60) ，癌旁组织为15%(3/20)，60例肝细胞癌高分化癌阳性表达率13%(2/15)，低分化癌为58%(26/45)，可见skp2在肝细胞癌中表达明显高于癌旁组织(Plt;0.05)，低分化癌明显高于高分化癌(Plt;0.01)。 结论 P27和skp2蛋白的表达情况与肝细胞癌的发生发展及恶性度有关。 【关键词】 肝细胞癌 P27 skp2 免疫组织化学 　　Abstract:　　Objective To explore the expression and significance of P27 and skp2 proteins in hepatocellular carcinoma (HCC). Methods Immunohistochemical(S-P) method was used to detect the expression of P27 and skp2 proteins in the 60 patients with HCC and 20 cases of peri-cancer tissues. Results The positive rate of P27 protein in 60 cases of hepatocellular carcinoma was 23% , that in the adjacent tumor tissue was 80% , The positive rate of p27 protein expression was significantly lower in tumor than in adjacent tumor tissue. The expression of p27 protein becomes lower with the increase in grading. The positive rate of skp2 protein in 60 cases of hepatocellular carcinoma was 47% , that in the adjacent tumor was 15% , The positive rate of skp2 protein expression was significantly higher in tumor than that in adjacent tumor tissue(Plt;0.05), lower grade differentiation was significantly higher than high grade differentitiation (Plt;0.01).Conclusions Expression of p27 and skp2 protein is closely correlated to the occurrence, development and malignancy degree of hepatocellular carcinoma. 　　Keywords:hepatocellular carcinoma; protein p27; protein skp2; immunohistochemistry 　　p27是skp2降解的靶蛋白，关于p27和skp2在肝细胞癌中的表达研究甚少，本文检测60例肝细胞癌p27和skp2蛋白的表达情况，以探讨它们在肝细胞癌中的作用及临床病理意义。 　　1　材料与方法 　　1.1　标本来源　收集我院2004.8-2007.8手术切除，经病理证实的60例肝细胞癌标本，患者年龄(18-66)岁，其中男性42例，女性18例。术前均未行化疗和放疗，其中高分化癌15例，低分化癌45例，同时取20例癌旁组织作对照。所有标本均为中性福尔马林固定，石蜡包埋，4μm厚切片。 　　1.2　免疫组织化学　操作步骤按免疫组化试剂盒说明进行，用已知的乳腺癌阳性片作为阳性对照，以PBS代替一抗作为阴性对照，DAB显色，苏木素复染，中性树胶封片。 　　1.3　结果判定标准　p27和skp2判定标准参考文献[1]以细胞核出现棕黄色颗粒沉