antitumor effect of a polypeptide fraction from arca subcrenata in vitro and in vivo抗肿瘤作用的多肽分数毛蚶体外和体内.pdfVIP

Mar. Drugs 2012, 10, 2782-2794; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Antitumor Effect of a Polypeptide Fraction from Arca subcrenata in Vitro and in Vivo Xianjing Hu 1,†, Liyan Song 2,*, Lijiao Huang 1,†, Qin Zheng 1 and Rongmin Yu 1,2,* 1 Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China; E-Mails: huxj2003@163.com (X.H.); excellen163.com (L.H.); zhengqin027@163.com (Q.Z.) 2 College of Pharmacy, Jinan University, Guangzhou 510632, China † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: tyrm@ (R.Y.); tsly@ (L.S.); Tel.: +86-20(R.Y.); Fax: +86-20(R.Y.); Tel.: +86-20(L.S.); Fax: +86-20(L.S.). Received: 18 October 2012; in revised form: 20 November 2012 / Accepted: 29 November 2012 / Published: 11 December 2012 Abstract: Arca subcrenata Lischke is a marine traditional Chinese medicine. The study investigated the antitumor effects of P2, a polypeptide fraction from A. subcrenata, and its toxicity in vitro and in vivo. The results showed that P2 could inhibit the proliferation of seven tumor cell lines, especially in HeLa and HT-29 cell lines. The IC50 values were 11.43 μg/mL for HeLa and 13.00 μg/mL for HT-29 treated by P2 for 48 h. P2 had little cytotoxicity on normal liver cells (L-02). The maximum tolerated dose (MTD) of P2 on KM mice was 1000 mg/kg by i.p. or i.v. The tu