analysis of regulatory network involved in mechanical induction of embryonic stem cell differentiation分析监管网络参与机械诱导胚胎干细胞分化.pdfVIP

Analysis of Regulatory Network Involved in Mechanical Induction of Embryonic Stem Cell Differentiation 1,2 3 2 2,3,4,5 2,3 Xinan Zhang , Maria Jaramillo , Satish Singh , Prashant Kumta , Ipsita Banerjee * 1 School of Mathematics and Statistics, Central China Normal University, Wuhan, China, 2 Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 3 Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 4 Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America, 5 Center for Complex Engineered Multifunctional Materials, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America Abstract Embryonic stem cells are conventionally differentiated by modulating specific growth factors in the cell culture media. Recently the effect of cellular mechanical microenvironment in inducing phenotype specific differentiation has attracted considerable attention. We have shown the possibility of inducing endoderm differentiation by culturing the stem cells on fibrin substrates of specific stiffness [1]. Here, we analyze the regulatory network involved in such mechanically induced endoderm differentiation under two different experimental configurations of 2-dimensional and 3-dimensional culture, respectively. Mouse embryonic stem cells are differentiated on an array of substrates of varying mechanical properties and analyzed for relevant endoderm markers. The experimental data set is further analyzed for identification of co-regulated transcription factors across different sub