analysis of microrna expression in the prepubertal testis分析微rna表达的青春期前的睾丸.pdfVIP

Analysis of MicroRNA Expression in the Prepubertal Testis 1,2. 4. 5 4 Gregory M. Buchold , Cristian Coarfa , Jong Kim , Aleksandar Milosavljevic , Preethi H. Gunaratne1,5, Martin M. Matzuk1,2,3* 1 Department of Pathology, Baylor College of Medicine, Houston, Texas, United States of America, 2 Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, United States of America, 3 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America, 4 Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, United States of America, 5 Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States of America Abstract Only thirteen microRNAs are conserved between D. melanogaster and the mouse; however, conditional loss of miRNA function through mutation of Dicer causes defects in proliferation of premeiotic germ cells in both species. This highlights the potentially important, but uncharacterized, role of miRNAs during early spermatogenesis. The goal of this study was to characterize on postnatal day 7, 10, and 14 the content and editing of murine testicular miRNAs, which predominantly arise from spermatogonia and spermatocytes, in contrast to prior descriptions of miRNAs in the adult mouse testis which largely reflects the content of spermatids. Previous studies have shown miRNAs to be abundant in the mouse testis by postnatal day 14; however, through Next Generation Sequencing of testes from a B6;129 background we found abundant earlier expression of miRNAs and describe shifts in the miRNA signature during this period. We detect