antifungal testing and high-throughput screening of compound library against geomyces destructans, the etiologic agent of geomycosis (wns) in bats抗真菌检测和高通量筛选化合物库的反对这种致病菌,geomycosis的病原体(白鼻综合症)的蝙蝠.pdfVIP

Antifungal Testing and High-Throughput Screening of Compound Library against Geomyces destructans, the Etiologic Agent of Geomycosis (WNS) in Bats 1,2 1 1 3 3 Sudha Chaturvedi , Sunanda S. Rajkumar , Xiaojiang Li , Gregory J. Hurteau , Michael Shtutman , Vishnu Chaturvedi1,2* 1 Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, United States of America, 2 Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York, United States of America, 3 Ordway Research Institute, Albany, New York, United States of America Abstract Bats in the northeastern U.S. are affected by geomycosis caused by the fungus Geomyces destructans (Gd). This infection is commonly referred to as White Nose Syndrome (WNS). Over a million hibernating bats have died since the fungus was first discovered in 2006 in a cave near Albany, New York. A population viability analysis conducted on little brown bats (Myotis lucifugus), one of six bat species infected with Gd, suggests regional extinction of this species within 20 years. The fungus Gd is a psychrophile (‘‘cold loving’’), but nothing is known about how it thrives at low temperatures and what pathogenic attributes allow it to infect bats. This study aimed to determine if currently available antifungal drugs and biocides are effective against Gd. We tested five Gd strains for their susceptibility to antifungal drugs and high-throughput screened (HTS) one representative strain with SpectrumPlus compound library containing 1,920 compounds. The results indicated that Gd is susceptible to a number of antifungal drugs at concentrations similar to the susceptibility range of human p