antifungal activity of (kw)n or (rw)n peptide against fusarium solani and fusarium oxysporum抗真菌活性(千瓦)n或(rw)n肽对腐皮镰刀菌和尖孢镰刀菌.pdfVIP

Int. J. Mol. Sci. 2012, 13, 15042-15053; doi:10.3390/ijms131115042 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Antifungal Activity of (KW) or (RW) Peptide against n n Fusarium solani and Fusarium oxysporum Ramamourthy Gopal 1, Hyungjong Na 2, Chang Ho Seo 3 and Yoonkyung Park 1,2,* 1 Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea; E-Mail: ramagopa@ 2 Department of Biotechnology, Chosun University, Gwangju 501-759, Korea; E-Mail: naozhong@ 3 Department of Bioinformatics, Kongju National University, Kongju 314-701, Korea; E-Mail: chseo@kongju.ac.kr * Author to whom correspondence should be addressed; E-Mail: y_k_park@chosun.ac.kr; Tel.: +82-62-230-6854; Fax: +82-62-225-6758. Received: 16 July 2012; in revised form: 14 August 2012 / Accepted: 17 October 2012 / Published: 15 November 2012 Abstract: The presence of lysine (Lys) or arginine (Arg) and tryptophan (Trp) are important for the antimicrobial effects of cationic peptides. Therefore, we designed and synthesized a series of antimicrobial peptides with various numbers of Lys (or Arg) and Trp repeats [(KW and RW) -NH , where n equals 2, 3, 4, or 5]. Antifungal activities of these peptides n 2 increased with chain length. Light microscopy demonstrated that longer peptides (n = 4, 5)