antigen delivery to macrophages using liposomal nanoparticles targeting sialoadhesincd169抗原交付使用脂质体纳米颗粒目标sialoadhesincd169巨噬细胞.pdfVIP

Antigen Delivery to Macrophages Using Liposomal Nanoparticles Targeting Sialoadhesin/CD169 1 1 1 1 1 2 Weihsu C. Chen , Norihito Kawasaki , Corwin M. Nycholat , Shoufa Han , Julie Pilotte , Paul R. Crocker , James C. Paulson1* 1 Departments of Chemical Physiology and Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America, 2 Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, United Kingdom Abstract Sialoadhesin (Sn, Siglec-1, CD169) is a member of the sialic acid binding Ig-like lectin (siglec) family expressed on macrophages. Its macrophage specific expression makes it an attractive target for delivering antigens to tissue macrophages via Sn-mediated endocytosis. Here we describe a novel approach for delivering antigens to macrophages using liposomal nanoparticles displaying high affinity glycan ligands of Sn. The Sn-targeted liposomes selectively bind to and are internalized by Sn-expressing cells, and accumulate intracellularly over time. Our results show that ligand decorated liposomes are specific for Sn, since they are taken up by bone marrow derived macrophages that are derived from wild type but not Sn2/ 2 mice. Importantly, the Sn-targeted liposomes dramatically enhance the delivery of antigens to macrophages for presentation to and proliferation of antigen-specific T cells. Together, these data provide insights into the potential of cell-specific targeting and delivery of antigens to intracellular organelles of macrophages using Sn-ligand decorated liposomal nanoparticles. Citation: Chen WC, Kawasaki N, Nycholat CM, Han S, Pilotte