an ultrasensitive electrochemical immunosensor for alpha-fetoprotein using an envision complex-antibody copolymer as a sensitive label甲胎蛋白的超灵敏的电化学免疫传感器使用一个设想complex-antibody共聚物作为敏感标签.pdfVIP

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an ultrasensitive electrochemical immunosensor for alpha-fetoprotein using an envision complex-antibody copolymer as a sensitive label甲胎蛋白的超灵敏的电化学免疫传感器使用一个设想complex-antibody共聚物作为敏感标签.pdf

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an ultrasensitive electrochemical immunosensor for alpha-fetoprotein using an envision complex-antibody copolymer as a sensitive label甲胎蛋白的超灵敏的电化学免疫传感器使用一个设想complex-antibody共聚物作为敏感标签

Materials 2012, 5, 2757-2772; doi:10.3390/ma5122757 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Article An Ultrasensitive Electrochemical Immunosensor for Alpha-Fetoprotein Using an Envision Complex-Antibody Copolymer as a Sensitive Label Ping Xiong 1, Ning Gan 1,*, Yuting Cao 1, Futao Hu 1, Tianhua Li 1 and Lei Zheng 2,* 1 The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China; E-Mails: 519791696@ (P.X.); caoyuting@ (Y.C.); hufutao@ (F.H.); litianhua@ (T.L.) 2 Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China * Authors to whom correspondence should be addressed: E-Mails: ganning@ (N.G.); nfyyzl@163.com (L.Z.); Tel.: +86-574-876-099-83 (N.G.); +86-20-6164-2147 (L.Z); Fax: +86-574-876-001-14 (N.G.); +86-20-6278-7681 (L.Z). Received: 21 August 2012; in revised form: 15 November 2012 / Accepted: 6 December 2012 / Published: 11 December 2012 Abstract: A novel strategy is presented for sensitive detection of alfa-fetoprotein (AFP), using a horseradish peroxidase (HRP)-functionalized Envision antibody complex (EVC) as the label. The Envision-AFP signal antibody copolymer (EVC-AFP Ab2) was composed of a dextran amine skeleton anchoring more than 100 molecules of HRP and 15 molecules of secondary antibody, and acted as a signal tag in the immunosensor. The sensor was constructed using the following steps: Firs