an in vivo polymicrobial biofilm wound infection model to study interspecies interactions一个生物膜体内幼童腹壁薄弱伤口感染模型研究种间相互作用.pdfVIP

An In Vivo Polymicrobial Biofilm Wound Infection Model to Study Interspecies Interactions 1,2 3 3 3 1,2 1 Trevor Dalton , Scot E. Dowd , Randall D. Wolcott , Yan Sun , Chase Watters , John A. Griswold , Kendra P. Rumbaugh1,2* 1 Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, Texas, United States of America, 2 Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas, United States of America, 3 Research and Testing Laboratory, Lubbock, Texas, United States of America Abstract Chronic wound infections are typically polymicrobial; however, most in vivo studies have focused on monospecies infections. This project was designed to develop an in vivo, polymicrobial, biofilm-related, infected wound model in order to study multispecies biofilm dynamics and in relation to wound chronicity. Multispecies biofilms consisting of both Gram negative and Gram positive strains, as well as aerobes and anaerobes, were grown in vitro and then transplanted onto the wounds of mice. These in vitro-to-in vivo multi-species biofilm transplants generated polymicrobial wound infections, which remained heterogeneous with four bacterial species throughout the experiment. We observed that wounded mice given multispecies biofilm infections displayed a wound healing impairment over mice infected with a single-species of bacteria. In addition, the bacteria in the polymicrobial wound infections displayed increased antimicrobial tolerance in comparison to those in single species infections. These data suggest that synergistic interactions between different bacterial species in wounds may contribute to hea