allotransplanted neurons used to repair peripheral nerve injury do not elicit overt immunogenicityallotransplanted神经元用于修复周围神经损伤不引起明显的免疫原性.pdfVIP

Allotransplanted Neurons Used to Repair Peripheral Nerve Injury Do Not Elicit Overt Immunogenicity Weimin Liu1,2, Yi Ren1,2, Adam Bossert1,2, Xiaowei Wang1,2, Samantha Dayawansa1,2, Jing Tong1,2,3, 4 5 2,6 1,2 Xiaoshen He , Douglas H. Smith , Harris A. Gelbard , Jason H. Huang * 1 Department of Neurosurgery, University of Rochester, Rochester, New York, United States of America, 2 Center for Neural Development and Disease, University of Rochester, Rochester, New York, United States of America, 3 Department of Neurosurgery, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China, 4 Department of Neurosurgery, Xijing Hospital, Fourth Military University, Xi’an, China, 5 Center for Brain Injury and Repair, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, 6 Department of Neurology, University of Rochester, Rochester, New York, United States of America Abstract A major problem hindering the development of autograft alternatives for repairing peripheral nerve injuries is immunogenicity. We have previously shown successful regeneration in transected rat sciatic nerves using conduits filled with allogeneic dorsal root ganglion (DRG) cells without any immunosuppression. In this study, we re-examined the immunogenicity of our DRG neuron implanted conduits as a potential strategy to overcome transplant rejection. A biodegradable NeuraGenH tube was infused with pure DRG neurons or Schwann cells cultured from a rat strain differing from the host rats and used to repair 8 mm gaps in the sciatic nerve. We observed enhanced regeneration with allogeneic cells compared to empty conduits 16 we