alphacoronaviruses in new world bats prevalence, persistence, phylogeny, and potential for interaction with humansalphacoronaviruses在新世界蝙蝠患病率、持久性、发展史、潜力与人类互动.pdfVIP

Alphacoronaviruses in New World Bats: Prevalence, Persistence, Phylogeny, and Potential for Interaction with Humans 1 3 3 2 6 Christina Osborne , Paul M. Cryan , Thomas J. O’Shea , Lauren M. Oko , Christina Ndaluka , 5 1 4 6 Charles H. Calisher , Andrew D. Berglund , Mead L. Klavetter , Richard A. Bowen , Kathryn V. Holmes2, Samuel R. Dominguez1,2* 1 Department of Pediatrics, University of Colorado School of Medicine, Aurora, Colorado, United States of America, 2 Department of Microbiology, University of Colorado School of Medicine, Aurora, Colorado, United States of America, 3 U.S. Geological Survey, Fort Collins Science Center, Fort Collins, Colorado, United States of America, 4 Pinon Canyon Maneuver Site, Model, Colorado, United States of America, 5 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America, 6 Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, United States of America Abstract Bats are reservoirs for many different coronaviruses (CoVs) as well as many other important zoonotic viruses. We sampled feces and/or anal swabs of 1,044 insectivorous bats of 2 families and 17 species from 21 different locations within Colorado from 2007 to 2009. We detected alphacoronavirus RNA in bats of 4 species: big brown bats (Eptesicus fuscus), 10% prevalence; long-legged bats (Myotis volans), 8% prevalence; little brown bats (Myotis lucifugus), 3% prevalence; and western long-eared bats (Myotis evotis), 2% prevalence. Overall, juvenile bats were twice as likely to be positive for CoV RNA as adult bats. At two of the rural sampl