alpha hemolysin induces an increase of erythrocytes calcium a flim 2-photon phasor analysis approachα溶血素诱导增加红细胞钙这部电影2-photon相量分析方法.pdfVIP

Alpha Hemolysin Induces an Increase of Erythrocytes Calcium: A FLIM 2-Photon Phasor Analysis Approach 1,2 ´ 3,4 1 3 Susana Sanchez , Laura Bakas , Enrico Gratton , Vanesa Herlax * ´ 1 Laboratory for Fluorescence Dynamics, University of California Irvine, Irvine, California, United States of America, 2 Microscopy Unit, Fundacion CNIC-Carlos III, Centro ˜ ´ Nacional de Investigaciones Cardiovasculares, Madrid, Espana, 3 Instituto de Investigaciones Bioquımicas La Plata (INIBIOLP), CCT- La Plata, CONICET, Facultad de Ciencias ´ ´ Medicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina, 4 Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina Abstract a-hemolysin (HlyA) from Escherichia coli is considered as the prototype of a family of toxins called RTX (repeat in toxin), a group of proteins that share genetic and structural features. HlyA is an important virulence factor in E. coli extraintestinal infections, such as meningitis, septicemia and urinary infections. High concentrations of the toxin cause the lysis of several cells such as erythrocytes, granulocytes, monocytes, endothelial and renal epithelial cells of different species. At low concentrations it induces the production of cytokines and ap