multivariate phenotypic divergence due to the fixation of beneficial mutations in experimentally evolved lineages of a filamentous fungus多元的有益突变表型差异由于固定实验丝状真菌的进化谱系.pdfVIP

Multivariate Phenotypic Divergence Due to the Fixation of Beneficial Mutations in Experimentally Evolved Lineages of a Filamentous Fungus 1,2 . 1,3. 1,4 1 1 Sijmen E. Schoustra * , David Punzalan , Rola Dali , Howard D. Rundle , Rees Kassen 1 Department of Biology, University of Ottawa, Ottawa, Ontario, Canada, 2 Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands, 3 Royal Ontario Museum, Toronto, Ontario, Canada, 4 McGill University, Montreal, Quebec, Canada Abstract The potential for evolutionary change is limited by the availability of genetic variation. Mutations are the ultimate source of new alleles, yet there have been few experimental investigations of the role of novel mutations in multivariate phenotypic evolution. Here, we evaluated the degree of multivariate phenotypic divergence observed in a long-term evolution experiment whereby replicate lineages of the filamentous fungus Aspergillus nidulans were derived from a single genotype and allowed to fix novel (beneficial) mutations while maintained at two different population sizes. We asked three fundamental questions regarding phenotypic divergence following approximately 800 generations of adaptation: (1) whether divergence was limited by mutational supply, (2) whether divergence proceeded in relatively many (few) multivariate directions, and (3) to what degree phenotypic divergence scaled with changes in fitness (i.e. adaptation). We found no evidence that mutational supply limited phenotypic divergence. Divergence also occurred in all possible phenotypic di