有哪些信誉好的足球投注网站identification of dna binding motifs of the mycobacterium tuberculosis phopphor two-component signal transduction system鉴定结核分枝杆菌的dna结合主题phopphor双组分信号转导系统.pdfVIP
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identification of dna binding motifs of the mycobacterium tuberculosis phopphor two-component signal transduction system鉴定结核分枝杆菌的dna结合主题phopphor双组分信号转导系统
Identification of DNA Binding Motifs of the Mycobacterium tuberculosis PhoP/PhoR Two- Component Signal Transduction System 1. 2. 1. 3 1 * Mena Cimino , Christophe Thomas , Amine Namouchi , Sarah Dubrac , Brigitte Gicquel , Deshmukh N. Gopaul2 ´ ´ ´ ´ ´ ´ ´ ´ 1 Unite de Genetique Mycobacterienne, Institut Pasteur, Paris, France, 2 Unite de Plasticite du Genome bacterien, Institut Pasteur, CNRS UMR 3525, Paris, France, 3 Unite ´ ` ` de Recherche Biologie des Bacteries pathogenes a Gram-positif, Institut Pasteur, Paris, France Abstract Background: The Mycobacterium tuberculosis PhoP/PhoR two-component signal transduction system controls the expression of about 2% of the genome and plays a major role in pathogenicity. However, its regulon has not been well characterized. Methodology/Principal Findings: The binding site of PhoP transcription regulator was identified in the upstream regions of msl3, pks2, lipF and fadD21 genes, by using gene fusions, electrophoretic mobility shift assays and DNase I footprinting experiments. A consensus sequence for PhoP binding was deduced. It consists of two direct repeats, DR1/DR2, associated with a third repeat, DR3, important in
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