molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients分子检测多个新兴病原体在囊性纤维化患者痰.pdfVIP
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molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients分子检测多个新兴病原体在囊性纤维化患者痰
Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients 1 ´ 1 2 3 2 Fadi Bittar , Herve Richet , Jean-Christophe Dubus , Martine Reynaud-Gaubert , Nathalie Stremler , 2 1 1 Jacques Sarles , Didier Raoult , Jean-Marc Rolain * ´ ´ ´ ´ 1 Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), CNRS-IRD, UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la ´ ´ ´ ´ ˆ Mediterranee, Marseille, France, 2 Departement des Maladies Respiratoires, Centre de Ressources et de Competences pour la Mucoviscidose Enfants (CRCM), Hopital ´ ´ ˆ Timone, Marseille, France, 3 Departement des Maladies Respiratoires, Centre de Ressources et de Competences pour la Mucoviscidose Adultes (CRCM), Hopital Sainte Marguerite, Marseille, France Abstract Background: There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients. Methodology/Principal Findings: Standard microbiological culture and phenotypic identification of bacteria in sputa from CF patients have been compared to molecular methods by the use of 16S rDNA amplification, cloning and sequ
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