multivariate statistical analyses demonstrate unique host immune responses to single and dual lentiviral infection多元统计分析展示独特的单和双慢病毒感染的宿主免疫反应.pdfVIP

Multivariate Statistical Analyses Demonstrate Unique Host Immune Responses to Single and Dual Lentiviral Infection 1. 1. 2 3 3 Sunando Roy , Jennie Lavine , Francesca Chiaromonte , Julie Terwee , Sue VandeWoude , Ottar Bjornstad1,4, Mary Poss1,4* 1 Department of Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America, 2 Department of Statistics, The Pennsylvania State University, University Park, Pennsylvania, United States of America, 3 Department of Microbiology, Immunology, and Pathology, Colorado State University, Ft. Collins, Colorado, United States of America, 4 Fogarty International Center, National Institutes of Health, Bethesda, Maryland, United States of America Abstract Background: Feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) are recently identified lentiviruses that cause progressive immune decline and ultimately death in infected cats and humans. It is of great interest to understand how to prevent immune system collapse caused by these lentiviruses. We recently described that disease caused by a virulent FIV strain in cats can be attenuated if animals are first infected with a feline immunodeficiency virus derived from a wild cougar. The detailed temporal tracking of cat immunological parameters in response to two viral infections resulted in high-dimensional datasets containing variables that exhibit strong co-variation. Initial analyses of these complex data using univariate statistical techniques did not account for interactions among immunological response variables and therefore potentially obscured significant effects between infection state and immunological parameters. M