identification of changes in wheat (triticum aestivum l.) seeds proteome in response to anti–trx s gene识别小麦的变化(小麦l .).pdfVIP

Identification of Changes in Wheat (Triticum aestivum L.) Seeds Proteome in Response to Anti–trx s Gene 1,2 3 1 1 1 1 Hongxiang Guo , Huizhen Zhang , Yongchun Li , Jiangping Ren , Xiang Wang , Hongbin Niu , Jun Yin1* 1 National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, China, 2 College of Life Sciences, Henan Agricultural University, Zhengzhou, China, 3 College of Public Health, Zhengzhou University, Zhengzhou, China Abstract Background: Thioredoxin h (trx h) is closely related to germination of cereal seeds. The cDNA sequences of the thioredoxin s (trx s) gene from Phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. Transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance to pre-harvest sprouting. Methodology/Principal Findings: Through analyzing the differential proteome of wheat seeds between transgenic wheat and wild type wheat, the mechanism of transgenic wheat seeds having high resistance to pre-harvest sprouting was studied in the present work. There were 36 differential proteins which had been identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). All these differential proteins are involved in regulation of carbohydrates, esters, nucleic acid, proteins and energy metabolism, and biological stress. The quantitative real time PCR results of some differential proteins, such as trx h, heat shock protein 70,