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multiparametric analysis of cell-free dna in melanoma patientsmultiparametric颗粒黑色素瘤患者的dna的分析
Multiparametric Analysis of Cell-Free DNA in Melanoma Patients 1. 1 . 2 2 3 Francesca Salvianti , Pamela Pinzani * , Paolo Verderio , Chiara Maura Ciniselli , Daniela Massi , 4 4 1 1 Vincenzo De Giorgi , Marta Grazzini , Mario Pazzagli , Claudio Orlando ` 1 Department of Clinical Physiopathology, Clinical Biochemistry Unit, Universita di Firenze, Firenze, Italy, 2 Unit of Medical Statistics and Biometry, Fondazione IRCCS, ` Istituto Nazionale dei Tumori, Milano, Italy, 3 Division of Pathological Anatomy, Department of Critical Care Medicine and Surgery, Universita di Firenze, Firenze, Italy, ` 4 Department of Critical Care Medicine and Surgery, Dermatology Unit, Universita di Firenze, Firenze, Italy Abstract Cell-free DNA in blood (cfDNA) represents a promising biomarker for cancer diagnosis. Total cfDNA concentration showed a scarce discriminatory power between patients and controls. A higher specificity in cancer diagnosis can be achieved by detecting tumor specific alterations in cfDNA, such as DNA integrity, genetic and epigenetic modifications. The aim of the present study was to identify a sequential multi-marker panel in cfDNA able to increase the predictive capability in the diagnosis of cutaneous melanoma in comparison with each single marker alon
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