multiparametric analyses reveal the ph-dependence of silicon biomineralization in diatomsmultiparametric分析揭示的生物矿化硅ph-dependence硅藻.pdfVIP

Multiparametric Analyses Reveal the pH-Dependence of Silicon Biomineralization in Diatoms ´ 1 2 ´ 2 1 3 1,4 Vincent Herve , Julien Derr , Stephane Douady , Michelle Quinet , Lionel Moisan , Pascal Jean Lopez * ` ´ ` ` 1 Biomineralisation et Morphogenese, CNRS UMR-8189, Ecole Normale Superieure, Paris, France, 2 Laboratoire Matiere et Systemes Complexes, CNRS UMR 7057, ´ ´ ´ ` ´ Universite Paris Diderot, Paris, France, 3 Laboratoire Mathematiques Appliquees a Paris 5, CNRS UMR 8145, Universite Paris Descartes, Paris, France, 4 Laboratoire ´ d’Excellence ‘‘CORAIL: Les recifs coralliens face au changement global,’’ Evolution des Biomineralisations, UMR CNRS 7208-MNHN-UPMC-IRD 207, Paris, France Abstract Diatoms, the major contributors of the global biogenic silica cycle in modern oceans, account for about 40% of global marine primary productivity. They are an important component of the biological pump in the ocean, and their assemblage can be used as useful climate proxies; it is therefore critical to better understand the changes induced by environmental pH on their physiology, silicification capability and morphology. Here, we show that external pH influences cell growth of the ubiquitous diatom Thalassiosira weissflogii, and modifies intracellular silicic acid and biogenic silica contents per cell. Measurements at the single-cell