multiphoton multispectral fluorescence lifetime tomography for the evaluation of basal cell carcinomas多光子多光谱荧光寿命评价断层的基底细胞癌.pdfVIP

Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas 1,2. 1. 1. 1 1 1 Rakesh Patalay , Clifford Talbot , Yuriy Alexandrov , Martin O. Lenz , Sunil Kumar , Sean Warren , 1 1 ¨ 4 1 2 5 Ian Munro , Mark A. A. Neil , Karsten Konig , Paul M. W. French , Anthony Chu , Gordon W. H. Stamp , Chris Dunsby1,3* 1 Photonics Group, Department of Physics, Imperial College London, South Kensington Campus, London, United Kingdom, 2 Department of Dermatology, Imperial College Healthcare NHS Trust, London, United Kingdom, 3 Department of Medicine, Imperial College Healthcare NHS Trust, London, United Kingdom, 4 JenLab GmbH, Jena, Germany, 5 CRUK London Research Institute, London, United Kingdom Abstract We present the first detailed study using multispectral multiphoton fluorescence lifetime imaging to differentiate basal cell carcinoma cells (BCCs) from normal keratinocytes. Images were acquired from 19 freshly excised BCCs and 27 samples of normal skin (in ex vivo). Features from fluorescence lifetime images were used to discriminate BCCs with a sensitivity/ specificity of 79%/93% respectively. A mosaic of BCC fluorescence lifetime images covering .1 mm2 is also presented, demonstrating the potential for tumour margin delineation. Using 10,462 manually segmented cells from the image data, we quantify the cellular morphology and spectroscopic differences between BCCs and normal skin for the first time. Statistically significant increases were found in the fluorescence life