multiple analyses of g-protein coupled receptor (gpcr) expression in the development of gefitinib-resistance in transforming non-small-cell lung cancer蛋白偶联受体(gpcr)的多个分析表达gefitinib-resistance的发展改变非小细胞肺癌.pdfVIP

Multiple Analyses of G-Protein Coupled Receptor (GPCR) Expression in the Development of Gefitinib-Resistance in Transforming Non-Small-Cell Lung Cancer 1,2 2 2 3 2 Naoko Kuzumaki *, Atsuo Suzuki , Michiko Narita , Takahiro Hosoya , Atsumi Nagasawa , 2 4 5 6 1 Satoshi Imai , Kohei Yamamizu , Hiroshi Morita , Tsutomu Suzuki , Yohei Okada , Hirotaka 1 4 1 2 James Okano , Jun K. Yamashita , Hideyuki Okano , Minoru Narita * 1 Department of Physiology, Keio University, School of Medicine, Tokyo, Japan, 2 Department of Pharamacology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan, 3 Biological Systems Control Team, Biomedicinal Information Research Center, Tokyo, Japan, 4 Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan, 5 Faculty of Pharmaceutical Sciences, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan, 6 Department of Toxicology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan Abstract There is increasing evidence that functional crosstalk between GPCRs and EGFR contributes to the progression of colon, lung, breast, ovarian, prostate and head and neck tumors. In this study, we performed multiple analyses of GPCR expression in a gefitinib-resistant non-small cell lung cancer (NSCLC) cell line, H1975, which harbors an L858R/T790M mutation. To determine the expression profile of mRNAs encoding 384 GPCRs in normal human lung fibroblast (NHLF) and H1975 cells, a