identification and characterization of two functionally unknown genes involved in butanol tolerance of clostridium acetobutylicum识别和描述的两个功能未知的基因参与丁醇acetobutylicum梭状芽胞杆菌的宽容.pdfVIP

Identification and Characterization of Two Functionally Unknown Genes Involved in Butanol Tolerance of Clostridium acetobutylicum Kaizhi Jia, Yanping Zhang, Yin Li* Institute of Microbiology, Chinese Academy of Sciences, Beijing, China Abstract Solvents toxicity is a major limiting factor hampering the cost-effective biotechnological production of chemicals. In Clostridium acetobutylicum, a functionally unknown protein (encoded by SMB_G1518) with a hypothetical alcohol interacting domain was identified. Disruption of SMB_G1518 and/or its downstream gene SMB_G1519 resulted in increased butanol tolerance, while overexpression of SMB_G1518-1519 decreased butanol tolerance. In addition, SMB_G1518-1519 also influences the production of pyruvate:ferredoxin oxidoreductase (PFOR) and flagellar protein hag, the maintenance of cell motility. We conclude that the system of SMB_G1518-1519 protein plays a role in the butanol sensitivity/tolerance phenotype of C. acetobutylicum, and can be considered as potential targets for engineering alcohol tolerance. Citation: Jia K, Zhang Y, Li Y (2012) Identification and Characterization of Two Functionally Unknown Genes Involved in Butanol Tolerance of Clostridium acetobutylicum. PLoS ONE 7(6): e38815. doi:10.1371/journal.pone.0038815 Editor: Eric Cascales, Centre National de la Recherche Scientifique, France Received February 26, 2012; Accepted May 11, 2012; Published June 29, 2012 Copyright: 2012 Jia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by National Science Foundation of China , National Basic Research Program of