monitoring of xenograft tumor growth and response to chemotherapy by non-invasive in vivo multispectral fluorescence imaging异种移植肿瘤的生长和对化疗的反应的监测体内非侵入性的多光谱荧光成像.pdfVIP

Monitoring of Xenograft Tumor Growth and Response to Chemotherapy by Non-Invasive In Vivo Multispectral Fluorescence Imaging 1,2 2 1 1 3 Henrike Caysa , Stefan Hoffmann , Jana Luetzkendorf , Lutz Peter Mueller , Susanne Unverzagt , ¨ 2 1 Karsten Mader , Thomas Mueller * 1 Department of Internal Medicine IV (Oncology/Hematology), Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany, 2 Institute of Pharmacy, Martin-Luther- University Halle-Wittenberg, Halle/Saale, Germany, 3 Institute of Medical Epidemiology, Biostatistics and Informatics, Martin-Luther-University Halle-Wittenberg, Halle/ Saale, Germany Abstract A continuous monitoring of the whole tumor burden of individuals in orthotopic tumor models is a desirable aim and requires non-invasive imaging methods. Here we investigated whether quantification of a xenograft tumor intrinsic fluorescence signal can be used to evaluate tumor growth and response to chemotherapy. Stably fluorescence protein (FP) expressing cell clones of colorectal carcinoma and germ cell tumor lines were generated by lentiviral transduction using the FPs eGFP, dsRed2, TurboFP635, and mPlum. Applying subcutaneous tumor models in different experimental designs, specific correlations between measured total fluorescence intensity (FI) and the tumor volume (V) could be established. The accuracy of correlation of FI and V varied depending on the cell model used. The application of deep-red FP expressing xenografts (TurboFP635, mPlum) was observed to result in improved correlations. This was also reflected by the results of a performed error analysis. In a model of visceral growing mPlum