human herpesvirus-6ab binds to spermatozoa acrosome and is the most prevalent herpesvirus in semen from sperm donors人类herpesvirus-6ab结合精子顶体精子和精液是最普遍的疱疹病毒从精子捐献者.pdfVIP

Human Herpesvirus-6A/B Binds to Spermatozoa Acrosome and Is the Most Prevalent Herpesvirus in Semen from Sperm Donors 1 2 1 3 4 ¨ 1 Maja D. Kaspersen , Peter B. Larsen , Emil Kofod-Olsen , Jens Fedder , Jesper Bonde , Per Hollsberg * 1 Department of Biomedicine, Aarhus University, Aarhus, Denmark, 2 Cryos International Sperm Bank, Aarhus, Denmark, 3 Fertility Clinic, Odense University Hospital, Odense, Denmark, 4 Department of Pathology and Clinical Research Center, Copenhagen University Hospital Hvidovre, Hvidovre, Denmark Abstract An analysis of all known human herpesviruses has not previously been reported on sperm from normal donors. Using an array-based detection method, we determined the cross-sectional frequency of human herpesviruses in semen from 198 Danish sperm donors. Fifty-five of the donors had at least one ejaculate that was positive for one or more human herpesvirus. Of these 27.3% (n = 15) had a double herpesvirus infection. If corrected for the presence of multiple ejaculates from some donors, the adjusted frequency of herpesviruses in semen was 27.2% with HSV-1 in 0.4%; HSV-2 in 0.1%; EBV in 6.3%; HCMV in 2.7%; HHV-6A/B in 13.5%; HHV-7 in 4.2%, whereas none of the samples had detectable VZV or HHV-8. Subsequently, we examined longitudinally data on ejaculates from 11 herpesvirus-positive donors. Serial analyses revealed that a donor who tested positive for herpesvirus at one time point did not necessarily remain positive over time. For the most frequently found herpesvirus, HHV-6A/B, we examined its association with sperm. For HHV-6A/B PCR-positive semen samples, HHV-6A/B could be detected on the sperm by flow cytometry. Conversely, PCR-negative semen samp