high bias gas flows increase lung injury in the ventilated preterm lamb高偏压气体流动增加通风早产儿肺损伤羔羊.pdfVIP

High Bias Gas Flows Increase Lung Injury in the Ventilated Preterm Lamb 1,4 2 3 4 4 Katinka P. Bach *, Carl A. Kuschel , Stuart B. Hooper , Jean Bertram , Sue McKnight , 4 3 3 1,5 3 Shirley E. Peachey , Valerie A. Zahra , Sharon J. Flecknoe , Mark H. Oliver , Megan J. Wallace , Frank H. Bloomfield1,4,5,6 1 Liggins Institute, University of Auckland, Auckland, New Zealand, 2 Royal Women’s Hospital, Parkville, Victoria, Australia, 3 The Ritchie Centre, Monash Institute of Medical Research, Clayton, Victoria, Australia, 4 Newborn Services, Auckland City Hospital, Auckland, New Zealand, 5 National Research Centre for Growth and Development, Auckland, New Zealand, 6 Department of Paediatrics: Child and Youth Health, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand Abstract Background: Mechanical ventilation of preterm babies increases survival but can also cause ventilator-induced lung injury (VILI), leading to the development of bronchopulmonary dysplasia (BPD). It is not known whether shear stress injury from gases flowing into the preterm lung during ventilation contributes to VILI. Methods: Preterm lambs of 131 days’ gestation (term = 147 d) were ventilated for 2 hours with a bias gas flow of 8 L/min (n = 13), 18 L/min (n = 12) or 28 L/min (n = 14). Physiological parameters were measured continuously and lung injury was assessed by measuring mRNA expression of early injury response genes and by histological analysis. Control lung tissue was collected from unventilated age-matched fetuses. Data were