high density lipoprotein (hdl) promotes glucose uptake in adipocytes and glycogen synthesis in muscle cells高密度脂蛋白(hdl)促进葡萄糖摄取的脂肪细胞和肌肉细胞的糖原合成.pdfVIP

High Density Lipoprotein (HDL) Promotes Glucose Uptake in Adipocytes and Glycogen Synthesis in Muscle Cells 1,2. 1. 1 1 1 1 1 1 Qichun Zhang , Yun Zhang , Haihua Feng , Rui Guo , Lai Jin , Rong Wan , Lina Wang , Cheng Chen , Shengnan Li1* 1Jiangsu Provincial Key Lab of Cardiovascular Diseases and Molecular Intervention, Department of Pharmacology, Nanjing Medical University, Nanjing, People’s Republic of China, 2 School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China Abstract Background: High density lipoprotein (HDL) was reported to decrease plasma glucose and promote insulin secretion in type 2 diabetes patients. This investigation was designed to determine the effects and mechanisms of HDL on glucose uptake in adipocytes and glycogen synthesis in muscle cells. 3 Methods and Results: Actions of HDL on glucose uptake and GLUT4 translocation were assessed with 1-[ H]-2- deoxyglucose and plasma membrane lawn, respectively, in 3T3-L1 adipocytes. Glycogen analysis was performed with amyloglucosidase and glucose oxidase-peroxidase methods in normal and palmitate-treated L6 cells. Small interfering RNA was used to observe role of scavenger receptor type I (SR-BI) in glucose uptake of HDL. Corresponding signaling molecules were detected by immunoblotting. HDL stimulated glucose uptake in a time- and concentration-dependent manner in 3T3- L1 adipocytes. GLUT4 translocation was significantly increased by HDL. Glycogen deposition got enhanced in L6 muscle cells paralleling w