high bone sialoprotein (bsp) expression correlates with increased tumor grade and predicts a poorer prognosis of high-grade glioma patients高骨涎蛋白(bsp)的表达与肿瘤分级和预测预后差增加高档神经胶质瘤患者.pdfVIP

High Bone Sialoprotein (BSP) Expression Correlates with Increased Tumor Grade and Predicts a Poorer Prognosis of High-Grade Glioma Patients 1. 1. 1 1 1 3,4 3,4 3,4 Tao Xu , Rong Qin , Jinxu Zhou , Yong Yan , Yicheng Lu , Xiaoping Zhang , Da Fu , Zhongwei Lv , 2 2 1 1 1 Weiqing Li , Chunyan Xia , Guohan Hu , Xuehua Ding , Juxiang Chen * 1 Department of Neurosurgery, Shanghai Institute of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, China, 2 Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai, China, 3 Department of Nuclear Medicine, Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai, China, 4 The Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences/Shanghai JiaoTong University School of Medicine, Shanghai, China Abstract Objectives: To investigate the expression and prognostic value of bone sialoprotein (BSP) in glioma patients. Methods: We determined the expression of BSP using real-time RT-PCR and immunohistochemistry in tissue microarrays containing 15 normal brain and 270 glioma samples. Cumulative survival was calculated by the Kaplan-Meier method and analyzed by the log-rank test. Univariate and multivariate analyses were performed by the stepwise forward Cox regression model. Results: Both BSP mRNA and protein levels were significantly elevated in high-grade glioma tissues compared with those of normal brain and low-grade glioma tissues, and BSP expression positively correlated with tumor gra