glutamatergic and gabaergic metabolism in mouse brain under chronic nicotine exposure implications for addictionglutamatergic和gaba ergic代谢在老鼠大脑慢性尼古丁暴露对成瘾的影响.pdfVIP

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glutamatergic and gabaergic metabolism in mouse brain under chronic nicotine exposure implications for addictionglutamatergic和gaba ergic代谢在老鼠大脑慢性尼古丁暴露对成瘾的影响.pdf

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glutamatergic and gabaergic metabolism in mouse brain under chronic nicotine exposure implications for addictionglutamatergic和gaba ergic代谢在老鼠大脑慢性尼古丁暴露对成瘾的影响

Glutamatergic and GABAergic Metabolism in Mouse Brain under Chronic Nicotine Exposure: Implications for Addiction ¤ Mohammad Shameem , Anant Bahadur Patel* NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology (CCMB), Council of Scientific and Industrial Research (CSIR), Hyderabad, India Abstract Background and Purpose: The effects of nicotine on cerebral metabolism and its influence on smoking behavior is poorly understood. An understanding of the chronic effects of nicotine on excitatory and inhibitory metabolic demand, and corresponding neurotransmission may provide clues for designing strategies for the optimal smoking cessation intervention. The objective of the current study was to investigate neuronal and astroglial metabolism in mice exposed to nicotine (0.5 and 2.0 mg/kg, sc) three times in a day for 4 weeks. Experimental Approach/Principal Findings: Metabolic measurements were carried out by co-infusing [U-13C ]glucose and 6 [2-13 13 1 13 13 1 C]acetate, and monitoring C labeling of amino acids in brain tissue extract using H-[ C] and C-[ H]-NMR spectroscopy. Concentration of 13C-labeled glutamate-C4 was increased significantly from glucose and acetate with chronic nicotine treatment indicating an increase in glucose oxidation by glutamatergic neurons in all brain regions and glutamate- glutamine neurotransmitter cycle in cortical and subcortical regions. However, chronic nicotine treatment led to increased labeling of GABA-C2 from glucose on