glycemic control promotes pancreatic beta-cell regeneration in streptozotocin-induced diabetic mice血糖控制促进糖尿病大鼠胰腺β细胞再生在体外实验.pdfVIP

Glycemic Control Promotes Pancreatic Beta-Cell Regeneration in Streptozotocin-Induced Diabetic Mice 1 1,3 1 1 2 2 Eric J. Grossman *, David D. Lee , Jing Tao , Raphael A. Wilson , Soo-Young Park , Graeme I. Bell , Anita S. Chong1 1 Department of Surgery, The University of Chicago, Chicago, Illinois, United States of America, 2 Department of Medicine, The University of Chicago, Chicago, Illinois, United States of America, 3 Department of General Surgery, Rush Medical Center, Chicago, Illinois, United States of America Abstract Background: Pancreatic beta-cells proliferate following administration of the beta-cell toxin streptozotocin. Defining the conditions that promote beta-cell proliferation could benefit patients with diabetes. We have investigated the effect of insulin treatment on pancreatic beta-cell regeneration in streptozotocin-induced diabetic mice, and, in addition, report on a new approach to quantify beta-cell regeneration in vivo. Methodology/Principal Findings: Streptozotocin-induced diabetic were treated with either syngeneic islets transplanted under the kidney capsule or subcutaneous insulin implants. After either 60 or 120 days of insulin treatment, the islet transplant or insulin implant were removed and blood glucose levels monitored for 30 days. The results showed that both islet transplants and insulin implants restored normoglycemia in the 60 and 120 day treated animals. However, only the 120- day islet and insulin implant groups maintained euglycemia (,200 mg/dl) following discontinuation of insulin treatment. The beta-cell was significantly increased in all the 120 day insulin-treated groups (insulin implant, 0.69 60.23 mg; and islet transplant, 0.91 60