global systems-level analysis of hfq and smpb deletion mutants in salmonella implications for virulence and global protein translation全球系统性分析hfq和smpb缺失突变体在沙门氏菌对毒性和全球蛋白质翻译的影响.pdfVIP

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global systems-level analysis of hfq and smpb deletion mutants in salmonella implications for virulence and global protein translation全球系统性分析hfq和smpb缺失突变体在沙门氏菌对毒性和全球蛋白质翻译的影响.pdf

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global systems-level analysis of hfq and smpb deletion mutants in salmonella implications for virulence and global protein translation全球系统性分析hfq和smpb缺失突变体在沙门氏菌对毒性和全球蛋白质翻译的影响

Global Systems-Level Analysis of Hfq and SmpB Deletion Mutants in Salmonella: Implications for Virulence and Global Protein Translation 1. 2. 4 3 1 Charles Ansong , Hyunjin Yoon , Steffen Porwollik , Heather Mottaz-Brewer , Brianne O. Petritis , 1 1 4 2 1 Navdeep Jaitly , Joshua N. Adkins , Michael McClelland , Fred Heffron , Richard D. Smith * 1 Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 2 Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon, United States of America, 3 Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 4 The Sidney Kimmel Cancer Center, San Diego, California, United States of America Abstract Using sample-matched transcriptomics and proteomics measurements it is now possible to begin to understand the impact of post-transcriptional regulatory programs in Enterobacteria. In bacteria post-transcriptional regulation is mediated by relatively few identified RNA-binding protein factors including CsrA, Hfq and SmpB. A mutation in any one of these three genes, csrA, hfq, and smpB, in Salmonella is attenuated for mouse virulence and unable to survive in macrophages. CsrA has a clearly defined specificity based on binding to a specific mRNA sequence to inhibit translation. However, the proteins regulated by Hfq and SmpB are not as clearly defined. Previous work identified proteins regulated by hfq using purification of the RNA-protein com