genome-wide transcriptional profiling reveals microrna-correlated genes and biological processes in human lymphoblastoid cell lines全基因组转录分析揭示了microrna-correlated人类lymphoblastoid细胞系基因和生物过程.pdfVIP

Genome-Wide Transcriptional Profiling Reveals MicroRNA-Correlated Genes and Biological Processes in Human Lymphoblastoid Cell Lines 1 2 2 2 2 Liang Wang *, Ann L. Oberg , Yan W. Asmann , Hugues Sicotte , Shannon K. McDonnell , Shaun M. 2 3 4 5 1 Riska , Wanguo Liu , Clifford J. Steer , Subbaya Subramanian , Julie M. Cunningham , James R. Cerhan2, Stephen N. Thibodeau1 1 Departments of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, United States of America, 2 Health Sciences Research, Mayo Clinic, Rochester, Minnesota, United States of America, 3 Department of Genetics, Stanley S. Scott Cancer Center, Louisiana State University, New Orleans, Louisiana, United States of America, 4 Departments of Medicine and Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, 5 Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America Abstract Background: Expression level of many genes shows abundant natural variation in human populations. The variations in gene expression are believed to contribute to phenotypic differences. Emerging evidence has shown that microRNAs (miRNAs) are one of the key regulators of gene expression. However, past studies have focused on the miRNA target genes and used loss- or gain-of-function approach that may not reflect natural association between miRNA and mRNAs. Methodology/Principal Findings: To examine miRNA regulatory effect on global gene expression under endogenous condition, we performed pair-wise correlation coefficient analysis on expression leve