有哪些信誉好的足球投注网站genetic characterization of type a enterotoxigenic clostridium perfringens strains遗传特性的a型产肠毒素的perfringens梭状芽胞杆菌菌株.pdfVIP
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genetic characterization of type a enterotoxigenic clostridium perfringens strains遗传特性的a型产肠毒素的perfringens梭状芽胞杆菌菌株
Genetic Characterization of Type A Enterotoxigenic Clostridium perfringens Strains 1. 1. 2 1 1 3 Agi Deguchi , Kazuaki Miyamoto *, Tomomi Kuwahara , Yasuhiro Miki , Ikuko Kaneko , Jihong Li , Bruce A. McClane3, Shigeru Akimoto1 1 Department of Microbiology, Wakayama Medical University School of Medicine, Wakayama, Japan, 2 Department of Bacteriology, The University of Tokushima Faculty of Medicine, Tokushima, Japan, 3 Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America Abstract Clostridium perfringens type A, is both a ubiquitous environmental bacterium and a major cause of human gastrointestinal disease, which usually involves strains producing C. perfringens enterotoxin (CPE). The gene (cpe) encoding this toxin can be carried on the chromosome or a large plasmid. Interestingly, strains carrying cpe on the chromosome and strains carrying cpe on a plasmid often exhibit different biological characteristics, such as resistance properties against heat. In this study, we investigated the genetic properties of C. perfringens by PCR-surveying 21 housekeeping genes and genes on representative plasmids and then confirmed those results by Southern blot assay (SB) of five genes. Furthermore, sequencing analysis of eight housekeeping genes and multilocus sequence typing (MLST) analysis were also performed. Fifty-eight C. perfringens strains were examined, including isolates from: food poisoning cases, human gastrointestinal disease cases, foods in Japan or the USA, or feces of healthy humans. In the PCR survey, eight of eleven
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