genetic characterization of smg-8 mutants reveals no role in c. elegans nonsense mediated decaysmg-8突变体的遗传特性揭示了在秀丽隐杆线虫废话没有作用介导的衰变.pdfVIP

Genetic Characterization of smg-8 Mutants Reveals No Role in C. elegans Nonsense Mediated Decay Jacqueline Rosains, Susan E. Mango* Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachussetts, United States of America Abstract The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use four independent assays to show that C. elegans smg-8 is not required to degrade nonsense-containing mRNAs. We examine the genetic requirement for smg-8 to destabilize the endogenous, natural NMD targets produced by alternative splicing of rpl- 7a and rpl-12. We test smg-8 for degradation of the endogenous, NMD target generated by unc-54(r293), which lacks a normal polyadenylation site. We probe the effect of smg-8 on the exogenous NMD target produced by myo-3::GFP, which carries a long 39 untranslated region that destabilizes mRNAs. None of these known NMD targets is influenced by smg-8 mutations. In addition, smg-8 animals lack classical Smg mutant phenotypes such as a reduced brood size or abnormal vulva. We conclude that smg-8 is unlikely to encode a component critical for NMD. Citation: Rosains J, Mango SE (2012) Genetic Characterization of smg-8 Mutants Reveals No Role in C. elegans Nonsense Mediated Decay. PLoS ONE 7(11): e49490. doi:10.1371/journal.pone.0049490 Editor: Georg Stoecklin, German Cancer Research Center, Germany Received June 5, 2012; Accepted October 12, 2012; Published November 16, 2012 Copyright: 2012 Rosains, Mango. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and sour