genomic alterations in breast cancer patients in betel quid and non betel quid chewers槟榔基因改变在乳腺癌患者和非槟榔嚼.pdfVIP

Genomic Alterations in Breast Cancer Patients in Betel Quid and Non Betel Quid Chewers 1 1 2 3 2 1 Mishi Kaushal , Ashwani K. Mishra , Jagannath Sharma , Eric Zomawia , Amal Kataki , Sujala Kapur , Sunita Saxena1* 1 National Institute of Pathology, ICMR, Safdarjung Hospital Campus, New Delhi, India, 2 Dr. B. Borrooah Cancer Institute, Guwahati, India, 3 Civil Hospital, Aizwal, India Abstract Betel Quid (BQ) chewing independently contributes to oral, hepatic and esophageal carcinomas. Strong association of breast cancer risk with BQ chewing in Northeast Indian population has been reported where this habit is prodigal. We investigated genomic alterations in breast cancer patients with and without BQ chewing exposure. Twenty six BQ chewers (BQC) and 17 non BQ chewer (NBQC) breast cancer patients from Northeast India were analyzed for genomic alterations and pathway networks using SNP array and IPA. BQC tumors showed significantly (P,0.01) higher total number of alterations, as compared with NBQC tumors, 48 617% versus 32625 respectively. Incidence of gain in fragile sites in BQC tumors were significantly (P,0.001) higher as compared with NBQC tumors, 34 versus 23% respectively. Two chromosomal regions (7q33 and 21q22.13) were significantly (p,0.05) associated with BQC tumors while two regions (19p13.3–19p12 and 20q11.22) were significantly associated with NBQC tumors. GO terms oxidoreductase and aldo-keto reductase activity in BQC tumors in contrast to G-protein coupled receptor protein signaling pathway and cell surface receptor linked signal transduction in NBQC tumors were enriched in DAVID. One network ‘‘Drug Metabolism, Molecular Transport, Nucleic Acid Metabolism’’ including genes AKR1B