genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast揭示了隐藏的表型变异的遗传基础平动readthrough增加酵母.pdfVIP

Genetic Basis of Hidden Phenotypic Variation Revealed by Increased Translational Readthrough in Yeast Noorossadat Torabi1,2, Leonid Kruglyak 1,3,4* 1 Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, United States of America, 2 Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America, 3 Department of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey, United States of America, 4 Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey, United States of America Abstract Eukaryotic release factors 1 and 3, encoded by SUP45 and SUP35, respectively, in Saccharomyces cerevisiae, are required for translation termination. Recent studies have shown that, besides these two key factors, several genetic and epigenetic mechanisms modulate the efficiency of translation termination. These mechanisms, through modifying translation termination fidelity, were shown to affect various cellular processes, such as mRNA degradation, and in some cases could confer a beneficial phenotype to the cell. The most studied example of such a mechanism is [PSI+], the prion conformation of Sup35p, which can have pleiotropic effects on growth that vary among different yeast strains. However, genetic loci underlying such readthrough-dependent, background-specific phenotypes have yet to be identified. Here, we used C653R sup35 , a partial loss-of-function allele of the SUP35 previously shown to increase readthrough of stop codons and recapitulate some [PSI+]-dependent phenotypes, to study the genetic basis of phenotypes revealed by increased translational readthrough in two divergent yeast strains: BY4724