genome-wide evaluation of histone methylation changes associated with leaf senescence in arabidopsis全基因组关联的组蛋白甲基化变化评价拟南芥叶片衰老.pdfVIP

Genome-Wide Evaluation of Histone Methylation Changes Associated with Leaf Senescence in Arabidopsis 1 1 2 3 Judy A. Brusslan *, Ana M. Rus Alvarez-Canterbury , Nishanth Ulhas Nair , Judd C. Rice , Michael J. Hitchler4, Matteo Pellegrini5 1 Department of Biological Sciences, California State University Long Beach, Long Beach, California, United States of America, 2 Laboratory for Computational Biology and ´ ´ Bioinformatics, School of Computer and Communication Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland, 3 Biochemistry and Molecular Biology, University of Southern California, Norris Cancer Center, Los Angeles, California, United States of America, 4 Radiation Oncology Department, Kaiser Permanente, Los Angeles, California, United States of America, 5 Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America Abstract Leaf senescence is the orderly dismantling of older tissue that allows recycling of nutrients to developing portions of the plant and is accompanied by major changes in gene expression. Histone modifications correlate to levels of gene expression, and this study utilizes ChIP-seq to classify activating H3K4me3 and silencing H3K27me3 marks on a genome- wide scale for soil-grown mature and naturally senescent Arabidopsis leaves. ChIPnorm was used to normalize data sets and identify genomic regions with significant differences in the two histone methylation patterns, and the differences were correlated to changes in gene expression. Genes that showed an increase in the H3K