健足颗粒对糖尿病皮肤溃疡大鼠血清NO ET-1水平影响.docVIP

健足颗粒对糖尿病皮肤溃疡大鼠血清NO ET-1水平影响收稿日期:2009-11-03 基金项目:浙江省中药现代化专项资金资助项目(浙财建字[2005]241号) 作者简介:黄在委(1977-),男,浙江杭州人,主治医师,研究方向:中医药治疗糖尿病及并发症的临床与实验研究。 通讯作者:柴可夫(1960-),男,浙江杭州人,主任医师、教授,博士研究生导师,博士,研究方向:中医药治疗糖尿病及并发症的临床与实验研究。 摘 要:目的:观察健足颗粒(加减防己黄芪汤)对糖尿病皮肤溃疡大鼠血管内皮功能的影响。方法:选用SD雄性大鼠90只,随机选取6只为正常对照组,其余大鼠以尾静脉注射四氧嘧啶40mg/kg,筛取符合糖尿病诊断标准的大鼠用于建立糖尿病皮肤溃疡模型,并随机分成6组,模型对照组、健足颗粒干预的高、中、低剂量组,中药对照组、西药对照组。实验结束后断头取血检测NO、ET-1含量以观察健足颗粒对血管内皮功能的影响。结果:与模型组比较,各治疗组大鼠血清NO含量均有不同程度升高,而血浆ET-1含量却有不同程度的降低,其中以健足颗粒高剂量组变化最明显(P0.05)。健足颗粒各剂量组的调节作用与剂量呈现相关性。结论:健足颗粒可通过升高血清NO含量、降低血浆ET-1水平来改善血管内皮功能,有效防治糖尿病皮肤溃疡发生发展。 关键词:糖尿病;皮肤溃疡;一氧化氮;内皮素-1;防治;健足颗粒;防己黄芪汤? 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)04-0837-03 Effects of JIANZU Granule on Nitric Oxide and Endothelin-1 in Rats with Diabetic Skin Ulceration (DSU) HUANG Zai?wei,CHAI Ke?fu,SONG Guang?ming,JIN Yi (Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China)) Abstract:?Objective?:To observe the effects of JIANZU Granule (Modified Fangji Huangqi Decoction) on vascular endothelial function of Rats with Diabetic Skin Ulceration (DSU).?Methods?:90 SD rats in male were randomized into two groups, namely Normal Control Group (NCG) with 6 rats, Model Group(MG) of 84 rats with tail vein injection of alloxan 40mg/Kg. Selecting the rats meeting the diagnostic criteria of Diabetes to establish Diabetic skin ulcer model for further observation, then randomly divided into 6 groups, Model Control Group(MCG), JIANZU Granule (JZG) High-dose Group, Middle-dose Group and Low-dose Group, Tang Maikang Control Group (TMCG), Metformin plus 654-2 Control Group(MNCG).In the end of the experiment, it was decapitated blood test NO, ET-1 to observe the effects of JZG on vascular endothelial function.?Results?:Compared with MCG, serum NO levels were increased to some degrees in all treatment groups, but the plasma ET-1 levels have different degrees of reduction. The most obvious change was observed in the High-dose Group (P0.05). The regulating funtion of each dose