壳聚糖对大鼠Ⅲ度烧伤创面表皮生长因子的影响研究.pdfVIP

壳聚糖对大鼠Ⅲ度烧伤创面表皮生长因子的影响研究 廖 红 ，董 志 ，傅洁民 ，朱 毅。，刘 全 ，杨佳丹 (1．重庆医科大学药理教研室／生物化学和分子药理 学重点实验室，重庆市 400016；2．重庆医药工业研究院，重庆市 400061；3．海南省药品检验所，海口市 570216) 中图分类号 R965 文献标识码 A 文章编号 1001—0408(2008)19—1467—03 摘 要 目的：研究壳聚糖对大鼠11I度烧伤创面表皮生长因子(EGF)@EGFmRNA表达的影响及意义。方法：105只Wistar大鼠 除5只作为正常对照组外，其余建立烧伤模型后分为壳聚糖低、中、高浓度(1％、2％、4％)组及贝复济组(阳性对照组)和自然愈合 组(模型组)，每日2次连续21 d于受伤处喷以相应药物，采用免疫组织化学法、核酸分子杂交法及图象分析法检测烧伤后3、7、14、 21 d时大鼠Ⅲ度烧伤创面EGF和EGFmRNA的表达。结果：与模型组比较，壳聚糖各浓度组均可增强EGF和EGFmRNA的表 达。并呈浓度依赖性，尤以4％壳聚糖组作用更强(P0．01或P0．05)。结论：壳聚糖对大鼠Ⅲ度烧伤创面有促愈合作用，其促愈 合机制与增强创面EGF和EGFmRNA表达有关。 关键词 壳聚糖；表皮生长因子；烧伤；创面愈合 Effect of Chitosan on Epidermal Growth Factor and Its mRNA in the Wound ofⅢ Degree in Rats with Skin Burn LIAO Hong，DONG Zhi，LIU Quan，YANG Jia—dan(Dept．of Pharmacology，Chongqing University of Medical Science，Key Lab of Biochemistry and Molecular Pharmacology，Chongqing 400016，China) FU Jie—min(Chongqing Pharmaceutical Industry Research Institute，Chongqing 400061，China) ZHU Yi(Hainan Provincial Institute for Drug Control，Haikou 570216，China) ABSTRACT OBJECTIVE：To study the effect of chitosan on the expression of epidermal growth factor (EGF)and its mRNA in rats with skin burn．METHODS：A total of 105 Wistar rats were enrolled：5 were assigned to normal control group； and 100 were established into burn mode1 and then divided into chitosan(1％，2％，and 4％)group，bFGF positive group，and model group (natural healing)．Each group was treated with corresponding drug b．i．d for 21 consecutive days on the burn surface．Immunohistochemisty，in situ hybridation and image analysis were employed to detect the expressions of EGF and its mRNA in rats withⅢdegree burn wound．RESULTS：As compared with model group，the expression of EGF and its mRNA in rats withⅢdegree burn wound was significantly increased in chitosan (1％，2％，and 4％)group in a concentration de— pendent manner，much as in 4％chitosan group(P0．01 or P0．05)．coNCLUSION：Chitosan could promote the wound healing ofⅢ