PPARα激动剂对高糖刺激的肾系膜细胞PEDF和VEGF mRNA表达的影响.pdfVIP

· 14· 交通医学2008年第22卷第 1期 Med J of Communications．2008．Vo1．22．No．1 [文章编号]1006—2440(2008)01—0014—04 PPARoL激动剂对高糖刺激的肾系膜细胞 PEDF和VEGF mRNA表达的影响 钟海花 明 扬 万 沁 徐 勇 (泸州医学院附属医院内分泌科； 神经外科，四川646000) 摘 要 目的：研究色素上皮细胞衍生因子(PEDF)和血管内皮生长因子(VEGF)在糖尿病。肾病中的作用以 及PPARa激动剂与糖尿病肾病的关系。方法：将培养的大鼠HBZY一1。肾小球系膜细胞株分为6组．高糖作为刺激 因子，PPARa激动剂WY14643作为干预因素。分别设正常组，高糖组，高糖加WY14643治疗组 (包括不同的 WY14643浓度)以及高糖加 WY14643溶剂对照组。用实时定量PCR法测定各组系膜细胞PEDF以及VEGF mRNA表达。结果：(1)与正常组相比较，高糖组肾系膜细胞PEDF mRNA的表达明显降低，而WY14643可逆转高 糖导致的肾系膜细胞PEDF mRNA表达的降低。(2)高糖组肾系膜细胞VEGF mRNA的表达较正常组明显增加。而 WY14643可减少高糖导致的。肾系膜细胞VEGF mRNA表达的增加。结论：高糖可抑制大鼠。肾系膜细胞PEDF并增 加VEGF的表达，而PPARa激动剂可在一定程度上逆转高糖导致的这种改变。 关键词 PPARa激动剂；糖尿病肾病；色素上皮细胞衍生因子：血管内皮生长因子 中图分类号 R584．2 文献标识码 A Effect of the PPAR-alpha agonist on the expression of PEDF and VEGF in cultured glomerular mesangial cells stimulated by high glucose ZHONG Haihua，MING Yang ，WAN Qin ，XU Yong(Department of Endocrinology； Department of Neurosurgery。The Affiliated Hospi— tal of Luzhou Medical College．Sichuan 646000) Abstract Objective：In order to explore the mechanism of pigment epithelium—derived factor(PEDF)and vascular endothelial growth factor(VEGF)in diabetic nephropathy and the relation between PPAR-alpha agonist and diabetic nephmpathy，the effect of high glucose and PPlAR—alpha agonist WY14643 on the expression of PEDF and VEGF in cuhured rat glomerular mesangial ceHs and discuss the relation between PPlAR—alpha agonist and diabetic nephropathy were investigated．To find new evidence for prevention and cure of diabetic nephropathy．Methods：Cuhured HBZY—l rat glomerular mesangial cells were divided into 6 groups，glucose was a stimulated factor，and PPAR—alpha agonist WY14643 was an intervenor：Normal Glucose group，High Glucose-treated grou