吸收促进剂对苦参碱体外吸收的影响及小鼠体内肝脏保护作用.pdfVIP

中圄兽科太擎学报 l16 Joureal of China Pharmaceutical University 2008，39(2)：1 16—121 Effect of enhancer on the absorption of matrine／n vitro and its hepato— protective effect on mice RUAN Li—ping，YU Bo—yang ，ZHU Dan—ni，RAN Qi—qiong Department of Chinese Medicinal Prescription，China Pharmaceutical University，Nanfing 210038，China Abstract Aim：T0 investigate the enhancement of enhancers on matrine n vitro and n vivo．Methods：The permeability of matrine across rat intestine was determined by non—everted intestinal sacs model in vitro．and the in vivo activity was assayed jn a model of acute liver iniury in mice．Sodium deoxycholate f SDC1 was selected as the enhancer candidate．Results：Among the tested absorption enhancers，only polysorbate 80(Tween 80)and SDC showed to be capable of increasing the transport of matrine through small intestinal mucosa in rats． Enhancement ratios of 1．6 was observed for 0．5％ SDC solutions with the best absorption enhancement for matrine in vitro，it was chosen to conduct hepatoprotective study in vivo．In mice with acute liver injury，combina— tion of matrine with 0．5％ SI)C demonstrated considerably more activity than matrine along after oral administra— tion．Conclusion：The results indicated that the absorption of matrine could be significantly improved by the ah— sorption enhancer． Key words matrine；absorption enhancer；hepatoprotection；HPLC CLC Number R944 Document code A Article lD l000—5048f2008102—0l l6—06 吸收促进剂对苦参碱体外吸收的影响及小鼠体内肝脏保护作用 阮丽萍，余伯阳 ，朱丹妮，冉启琼 (中圜药科大学中药复 研究窀，南京210038) 摘 要 目的：研究比较不同吸收促进剂对苦参碱体内外吸收作用的影响。方法：苦参碱于大鼠小肠的体外渗透速 率由非翻转小肠囊法测定，其体内活性通过小鼠急性肝损伤模型评价。结果：在受试的吸收促进剂中，只有 rween 80和脱 氧胆酸钠(SDC)能够增加苦参碱在大鼠小肠黏膜的渗透量． 0．5％SDC的体外吸收促进作用最好，增渗比为1．6 其体内 活性的评价显示，对于急性肝损伤模型小鼠，服用舍0．5％SDC的苦参碱溶液较不含吸收促进剂的苦参碱溶液体内活性明 显提高．、结论：吸收促进刑能明显改善苦参碱的体内外吸收 关键词 苦参碱；吸收促进剂；肝脏保护：高效液相色谱法 Matrin