竹康宁中竹叶总黄酮的提取及测定方法研究.pdfVIP

竹康宁中竹叶总黄酮的提取及测定方法研究 宫维娜1，于惠春2，陈伟h (1．烟台经济技术开发区卫生防疫站，烟台264006； 2．烟台张裕葡萄酿酒股份有限公司，烟台264000) 摘要：霸的：研究竹康宁中竹叶总黄酮的提取及测定方法。方法：在单因素试验的基础上，采用 k(34)正交设计对乙醇网流提取法和硝酸铝比色测定法进行试验。结果：确定了乙醇回流提取法的最佳提取 王艺是：为％乙醇，辩液我1：30，90℃霾流提取2次，每次2h。逮色浃溺定3裁次锋康宁孛黪镗跨黄醺。鬻 行，结果令人满意。 关键词：竹康宁；竹砖黄酮；提驭；测定；比色法 中巨分类鼍：TS202．1 文献标识码：A 文拳编号：1006—2513f2008)Ol—0164一03 onthemethodsofextractanddetermine Study ofbambooflavonefrOmbamboo feafessence GONG Weil‘ Wei—nal，髑Hui-chun2，CHEN Ecnomic Zoon Teehnoic (1．Yantai DevelopmentHygieneEpidemicStation，Yantai264006； 2。Yantai PioneerWine Changyu Co．，LTD。，Yantai264006) onthemethodsofextractanddetermineofbamboo frombamboo Abstract：0bjective：Tostudy flavone leaf thebasicofmonofactorialmethodsofextractanddeterminebamboo from e粼nee．Methods：On tests，the of flavone bambooleafessencewerestudiedthe best examination．Results：弧eethanolcircumfluence麟- by k(34)orthogonal tractionconditions extractionfor were船f010ws：leaves：extraction twiceat90℃and2hourseachtime．Threebatchsbambooleaf燃8cnceweredetermined recoveries byeolorimetry．强e werebetween88．0％and92．0％andtheRSDwerebetweenl，3％and2．2％．necontentofbamtxoflavonoidsin themWerebetween5．1％and result8a∞satisfiedandthemethods with 5．3％．Conclusuons：The to