肿瘤干细胞标记物在肺癌中的诊断价值和临床意义.pdfVIP

关键词：Musashi1，肿瘤干细胞标志物，纤支镜活检, 肺癌 - 2 - Expression of some SCS in tissues obtained by fiberoptic bronchoscopy and its diagnostic value of lung cancer ABSTRACT Objective: Comparative analysis the expression level of tumor stem cells markers such as CD133，CD44，Musashi1 ，Musashi2 ，OCT4， Nanog ，Sox2，Bmi-1 gene in biopsy samples of fiberoptic bronchoscopy(FB) in patients with lung cancer , and research whether the diagnostic value exists in Cancer stem cells in lung cancer ,then to explore which stem cell markers could be served as novel molecular for human lung cancer.and evaluate its diagnostic value of lung cancer . Methods : Reverse transcription polymerase chain reaction (RT-PCR) was used to examine every gene for biopsy samples of FB in 91 patients with lung cancer and 27 patients with benign pulmonary disease .Results: ①Musashi1, Nanog, Musashi2, in the expression of lung cancer significantly higher than the control group(P 0.05), the expression of Bmi1, Oct4, Sox2, CD133, CD44 in the experimental group and control group is no significant difference (P 0.05); ②The specificity and sensitivity of Musashi1 in lung cancer was highest (P 0.05).③No significant correlations were found between the expression of Musashi1 and clinical pathological indexes including age , gender , smoking , occupation ,the tumor size and pathological types of cancer(P ＞0.05). ④The - 3 - gray degree ratios of the Musashi1 mRNA belt to the β-actin mRNA belt varied accordingly to different pathologic grades and degree of differentiation of cancer .There were significant difference between them(P＜0.05). The positive