基于L-FABP：GFP转基因斑马鱼构建放射性肝损伤模型的初步探讨.pdfVIP

医药前沿 2015年8月 第5卷第24期 论 著 基于 L-FABP:GFP 转基因斑马鱼构建放射性 肝损伤模型的初步探讨 胡威 （遵义医学院附属医院胸部肿瘤科 贵州 遵义 563003） 【摘要】 目的：初步探讨以 L-FABP10:GFP 肝组织特异性转基因斑马鱼建立放射性肝损伤模型的可能性。方法：出生后 5 天的斑 马鱼，给予“剂量爬坡梯度”放疗，连续观察 5 天，了解幼鱼放疗安全性及安全剂量；结合“剂量爬坡”试验的结果及临床实践， 选择合适的剂量进行放疗，连续观察 5 天，了解放疗后斑马鱼肝脏荧光强度、面积和形态的变化。结果：“剂量爬坡”试验显示：0?? 2、6、10、20、30、40Gy/ 次条件下的放射处理对斑马鱼是安全的；接近临床实践剂量 40Gy/ 次的放疗处理未见斑马鱼肝脏荧光强度、 范围及形态的变化。结论：L-FABP:GFP 转基因斑马鱼放疗后 54h 内无法通过监测肝 GFP 荧光变化了解放射性肝损伤情况，该模型的 建立仍需要进一步探讨。 【关键词】 放疗；斑马鱼；肝损伤；模型 【中图分类号】R735.7 【文献标识码】A 【文章编号】2095-1752（2015）24-0017-03 Establishment of Radiation-induced Liver Injury Model with LFABP:GFP Transgene Zebrafish Hu Wei。Department of Thoracic Cancer, Affiliated Hospital of Zunyi Medical College, Guizhou Province, Zunyi 563003,China 【Abstract】Objective To investigate the establishment of radiation-induced liver injury model with L-FABP:GFP transgene zebrafish. Methods 5 days post-birth zebrafishes were given Dose climing test radiation and to assess the radiational security and choose the safe doses 5 days later. Combinded the above results with clinical practices to choose appropriated radiational doses, continuously observed the strength, area and shape changes of fluorescence for 54 h after radiation. Results Dose climing test show that it is safe to radiate under 0，2, 6, 10, 20, 30, 40 Gy/F for Zebrafish. There were no significant strength, area and shape changes of fluorescence under the doses near to clinical practice dose (40 Gy/F). Conclusion The radiation-induced liver injury of L-FABP:GFP transgene zebrafish can’t be observed