共代谢条件下丁基黄药的生物降解实验研究_鄢恒珍.pdfVIP

鄢恒珍等　共代谢条件下丁基黄药的生物降解实验研究 共代谢条件下丁基黄药的生物降解实验研究＊ 1,2 1 # 1 1 1 鄢恒珍 　龚文琪 　梅光军 　陈绍华 　陈晓东 (1.武汉理工大学资源与环境工程学院, 湖北　武汉430070;2.湖南城市学院城市建设系, 湖南　益阳413000) 　　摘要　微生物共代谢是降解废水中难降解有机物的重要方式。通过实验研究, 比较了以丁基黄药作为唯一碳源和能源以及 有共代谢基质存在下丁基黄药的降解过程。结果表明, 共代谢显著提高了丁基黄药的降解率。在25 ℃条件下, 以丁基黄药为唯一 碳源和能源时, 其降解率仅为43.1%;而利用葡萄糖作为共代谢基质, 当葡萄糖质量浓度为0.20 g/L 时, 振荡培养72 h 后丁基黄药 的降解率可达65.2%;再加入微量的蛋白胨作为氮源后, 丁基黄药的降解率可提高到73.5%;加入共代谢基质后微生物的适应期由 原来的36 h 缩短至24 h。共代谢是提高丁基黄药生物降解性能的一条有效途径。 　　关键词　丁基黄药　共代谢　生物降解 1,2 1 1 1 Study on co-metabolism biodegradation of butyl xanthate　Yan H engz hen , Gong Wenqi , Mei Guangj un , Chen Shaohua , 1 Chen Xiaodong .(1.S chool of Resources and Env ironmental Engineering, Wuhan Universit of Technolog , Wuhan H ubei 430070;2.D ep artment of Uban Cons truction, H unan Cit Universit ,Yi ang H unan 413000) Abstract:　The microorganism co-meta olism was an important mode for iodegrading the refractory organics in wastew ater.The processes of utyl xanthate iodegradation w ith and without the existence of co-meta olism su - st rates w ere studied and compared.The experimental results show ed that the presence of co-meta olism su strates significantly increased xanthate degradation.The degradation rate of utyl xanthate was 43.1% after shaking culture for 72 h at 25 ℃w ith utyl xanthate as the sole source of car on and energy.While the presence of additional car on source such as glucose, sucrose and amylum (0.20 g/L)increased the degradation rate of utyl xanthate to 65.2%, 59.4%, 56.4%under the same iodegradation condition, and the coexistence of nitrogen source (peptone)and car on source (glucose)increas