miR224在乳腺癌中的表达及其临床意义.docVIP

miR-224在乳腺癌中的表达及其临床意义 温州医学院附属三院肿瘤外科浙江省瑞安325200 】目的探讨miR-224在乳腺癌组织中的表达及其与临床病理 特征的关系。方法用荧光定量PCR技术检测55例乳腺癌患者的癌组织及其癌旁 的正常乳腺组织中miR-224的表达情况。结果miR-224在乳腺癌组织中的相对 表达量为0.321plusmn;0.324,在配对的正常乳腺组织的相对表达量为 0.227plusmn;0.370,两者的差异具有统计学意义(t=2.182, P=0.033lt;0.05), miR-224在乳腺癌中组织的表达与乳腺患者的肿块大小、淋巴结转移、病理分期 有关(P均lt;0.05),而与患者年龄、分化程度无关(P均gt;0.05)。结论miR-224 在乳腺癌中的表达升高，升高的miR-224提示乳腺癌患者的不良预后。 关键词】乳腺癌；miR-224；临床病理特征；预后 Expression and clinical significance of miR-224 and in human breast cancer.XUE Di-xin, CHEN Ji-xian, LIN Dao-zhe, YUming, WU Wei-li, LIANG Mei-zhen, HEXin-wei, UN Xiao, CHEN Ha-ha.Department of Surgical Oncology, The Third AffiliatedHospital, Wenzhou Medical College, Ruian325200，China.corresponding author： CHEN Ji-xian, : HYPERLINK mailto:xue-di-xin@163.comxue-di-xin@163.com [Abstract] Objective To explore the expression of miR-224 and their correlation with Clinicopathological characteristics in breastcancer.Methods Expression of miR-224 was detected by real-time quantitative PCR in 55 cases of breast cancer and their correspondingadjacent normal tissue.Results The relative expression level of miR-224 was 0.321plusmn;0.324 in breast cancer, and 0.227plusmn;0.370 in matchednormal tissue，significant differences were observed between them(t=2.182，P=0.033lt;0.05).ln breast carcinoma, the relative expression levelof miR-224 was associate with size, lymph node metastasis, pathological stage(Plt;0.05)，no significant differences were observed regardingage, the degree of differentiation.(Pgt;0.05).Conclusion The expression level of miR-224 was elevated in breast cancer, and overexpressionof miR-224 indicate poor prognostic. Key words】breast cancer； miR-224; Clinicopathological characteristics； prognostic. 】R737.9 】A 】2096-0867 (2015) -09-012-02 MicroRNA(miRNA)是一种小分子非编码RNA,近些年来，研究表明在乳 腺癌组织中miroRNA出现异常表达，如miR-125b、miR-21、miR-191 [1],并 且异常表达的micoRNA与乳腺癌细胞的增值、侵袭、转移相关[2-3],而miR-224 在乳腺癌患者的研究甚少，本实验研究miR-224在乳腺癌中的表达及其与临床 特征的关系。资料与方法 一般资料 收集温州医学院附属三院肿瘤外科2015年2月至2015年8月手术切 除的55例术前