MiR370在高频脉冲电磁场下对骨髓间充质干细胞调控作用的初步研究.docVIP

MicR-370在高频脉冲电磁场下对骨髓间充质干细胞调控作用的初步研究 郭杰苏伟黄钊谢能峰何肖丞 (广西医科大学第一附属医院创伤骨科屮心广西南宁530021) 】R的：通过检测gt;380MHz高频脉冲电磁场刺激不骨髓间充质干细屮 microRNA-370的表达变化规律，探讨mir-370对骨髓间充质干细胞可能的影响。 方法：采用贴壁筛选法分离培养SD人鼠骨髓间充质干细胞，取第3代细胞随机 分为两组：电磁场照射组、空白对照组，分别提取总RNA通过Realtime-PCR的 结果推测mir-370对大鼠间充质干细胞屮的bmp-7的调控作用。结果：经全骨髓 细胞贴壁筛选法分离培养的SD人鼠骨髓间充质干细胞符合人鼠骨髓间充质干细 胞基本特征。大鼠基因组模板BmP7已扩增出来，大小与预测一致。在转染克隆 有BMP7基因3rsquo;UTR质粒的实验组中,mimics组与空白组和NC组相比, (Pgt;0.05)无统计学差异，证明mir-370不能与BMP7基因3rsquo;UTR结合， 影响萤光素酶的活性。结论：mir-370不能负向调控bmp-7蛋白从而到达抑制骨 髓间充质干细胞的作用。 关键词】小分子RNA骨髓间充质干细胞高频脉冲电磁场调控定量聚合 酶链式反应 屮图分类号】R68 】B 】2095-1752 (2013) 10-0386-04 Abstract】 [Objective]:gt; 380MHz high-frequency pulsed electromagnetic fields(HF-PEMFs) stimulation by detecting bone marrow mesenchymal stem cells(BMSCs), microRNAs-370(mir-370) expression variation explore mir-370 on BMSCs may. [Methods]: adherence screening method is isolated and cultured SD rat BMSCs, the 3rd generation cells were randomly divided into two groups: the electromagnetic field radiation group, blank control group, respectively, total RNA was extracted by Realtime-PCR results speculate mir-370 regulation of bmp-7 in rat mesenchymal stem cells. [Results]: adherence screening method of whole bone marrow cells were isolated and cultured in SD rat BMSCs in line with the basic characteristics of rat BMSCs. The rat genome amplified template Bmp7 out, size and forecasts. Clone transfected the BMP7 gene 3UTR plasmid experimental group Mimics the best choice group compared with the control group and the NC group, (Pgt; 0.05) was not statistically different, and that mir-370 can not be combined with BMP7 gene 3UTR affect the activity of the luciferase. [Conclusion]: mir-370 is not suppression of BMSCs to reach the negative regulation of bmp-7 protein. Keywords】 small molecule RNA; Bone marrow mesenchymal stem cells; high-frequency pulsed electromagnetic fields; regulation;Quantitative polymerase chain reaction 引言 骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs)是存在 于骨髓基质系统中的一种组织干