孝扇草抗炎镇痛有效部位筛选研究.docVIP

孝扇草抗炎镇痛有效部位筛选研究 　　 摘 要：目的：分离并筛选孝扇草抗炎镇痛作用的有效部位。方法：采用系统溶剂法对孝扇草醇提物进行初步分离；采用二甲苯致小鼠耳廓肿胀实验，筛选孝扇草抗炎作用有效部位；采用热板法和醋酸致小鼠扭体法实验，筛选其镇痛作用有效部位。结果：孝扇草醇提乙酸乙酯萃取部位对二甲苯致小鼠耳廓肿胀的抑制率为50.53%，与空白对照组比较有显著性差异（P0.05）；孝扇草醇提乙酸乙酯萃取部位和各溶剂萃取剩余部位均能显著提高小鼠痛阈值，与空白对照组比较有显著性差异（P0.05，P0.05）；孝扇草醇提乙酸乙酯和正丁醇萃取部位均能明显减少醋酸致小鼠扭体反应次数，与空白组比较有显著性差异（P0.05，P0.05）。结论：孝扇草醇提乙酸乙酯萃取部位可能是其抗炎镇痛有效部位。 　　 关键词：孝扇草；有效部位；抗炎；镇痛 　　中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2012)01-0155-03 　　Study on Screening Valid Target of Anti-inflammatory and Analgesic Effets of Calystegia Soldanella 　　TAN Li，SHEN Qing，HUANG Zhen 　　(College of Pharmaceutical science，Zhejiang University of Traditional Chinese Medicine，Hangzhou 310053，Zhejiang，China) 　　Abstract:Objective:To separate and screen the valid target of anti-inflammatory and analgesic effects of Calystegia soldanella. Methods:Systematic solvent method is used to separate the ethanol extract of Calystegia soldanella，ear edema induced by dimethylbenzene in mice was used to screen the valid target of the anti-inflammatory effects，mice hot-plate procedures and mice wthithing induced by acetic acid were used to screen the valid target of the analgesic effects. Results:The inhibition ratio of swelling induced by dimethylbenzene in mice of the ethanol-extracted acetoacetate-abstraction group of Calystegia soldanella is 50.53% (P0.05). The acetoacetate-abstraction group and the rest group could significantly prolong the mouse hot-plate-induced pain of the pain threshold of the mice(P0.05，P0.05). The ethanol-extracted acetoacetate-abstraction group and the n-buOH-abstraction group can significantly decrease acetic acid-induced writhing response in mice(P0.05，P0.05).Conclusion:The ethanol-extracted acetoacetate-abstraction group of Calystegia soldanella is most propably the valid target of anti-inflammatory and analgesic. 　　Key words:Calystegia soldanella；valid target；anti-inflammatory；analgesic 　　 　　 孝扇草为旋花科（Convolvulaceae）打碗花属（Calystegia）植物肾叶打碗花（Calystegia soldanella (L.) R.Br.[Convolvulus s